Jl. Petrie et al., MOLECULAR CHARACTERIZATION OF ECHINOSTOMA-CAPRONI AND E-PARAENSEI BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA (RAPD) ANALYSIS, The Journal of parasitology, 82(2), 1996, pp. 360-362
Genomic DNA from Echinostoma caproni and Echinostoma paraensei was amp
lified by the polymerase chain reaction using primers (5'-TCGTAGCCAA a
nd 5'-TCACGATGCA), originally found to differentiate species and strai
n of Schistosoma. The 2 putative species of Echinostoma produced disti
nct banding patterns clearly distinguishable from one another, thereby
suggesting that RAPD (random amplification of polymorphic DNA) analys
is may be useful for the identification of echinostome strains and spe
cies previously misunderstood or undescribed, and that primers develop
ed for species within a given genus, e.g., Schistosoma, may have broad
er application in identifying other trematodes.