PURINE SUPPRESSION OF PROLIFERATION OF SERTOLI-LIKE TM4 CELLS IN CULTURE

The effect of adenosine and related compounds on the proliferation of cultured TM4 cells, a Sertoli-like cell line, has been examined. Adeno sine, as well as A1 and A2 adenosine receptor agonists (cyclohexyladen osine and imethoxyphenyl)-2-(2-methylphenyl)ethyl]adenosine) inhibited cell proliferation. These effects were prevented by 8-cyclopentyl the ophylline, 1,3-dimethyl-propargylxanthine and caffeine, antagonists at the A1, A2 and both receptors, respectively. The xanthines had no eff ect by themselves and, consistent with this, the bathing medium was fo und not to contain detectable levels of adenosine. It is concluded tha t TM4 cell proliferation can be regulated by receptors for adenosine.