MET-ENKEPHALIN INDUCES CYTOLYTIC PROCESSES OF APOPTOTIC TYPE IN K562 HUMAN ERYTHROID LEUKEMIA-CELLS

Authors
MERNENKO OA BLISHCHENKO EY MIRKINA II KARELIN AA
Citation
Oa. Mernenko et al., MET-ENKEPHALIN INDUCES CYTOLYTIC PROCESSES OF APOPTOTIC TYPE IN K562 HUMAN ERYTHROID LEUKEMIA-CELLS, FEBS letters, 383(3), 1996, pp. 230-232
Citations number
20
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
383
Issue
3
Year of publication
1996
Pages
230 - 232
Database
ISI
SICI code
0014-5793(1996)383:3<230:MICPOA>2.0.ZU;2-6
Abstract
Cytolytic activity of Met-enkephalin, an endogenous opioid peptide, wa s studied within the 10(-7)-10(-17) M concentration range in K562 huma n erythroid leukemia cells. Cytolytic activity was determined by the t rypan blue inclusion method after 13, 15 and 18 h of Met-enkephalin co -incubation with target cells. Discrete maxima of cytolytic activity w ere detected at concentrations of 10(-9)-10(-10), 10(-13) and 10(-15) M. Cytolysis was accompanied by internucleosomal DNA fragmentation whi ch is indicative of the mechanism of cell death being apoptosis.