F. Chanteloube et al., MICROPROPAGATION OF TULIPA-GESNERIANA L - REGENERATION OF BULBLETS ONGROWING FLORAL STEM SEGMENTS CULTURED IN-VITRO, Acta botanica gallica, 142(4), 1995, pp. 301-307
Explants were excised from 3 different regions of active growing flora
l stem of tulip, and were cultured in vitro on MS medium with growth r
egulators. Adventitious bud formation was highest in the median region
. Buds formed bulblets if a cold (5 degrees C) then a warm (20 degrees
C) period were applied. Bulblet production was promoted by a 3 months
cold treatment when compared to 1 or month treatment. Histological ob
servations indicated that the neoformed bulblets appeared to be functi
onal for further development in soil.