Jl. Kirkland et al., EFFECTS OF FAT DEPOT SITE ON DIFFERENTIATION-DEPENDENT GENE-EXPRESSION IN RAT PREADIPOCYTES, International journal of obesity, 20, 1996, pp. 102-107
Variability in physiological characteristics among adipose depots coul
d be caused, in part, by mechanisms intrinsic to cells comprising the
depots. To evaluate the contribution of intrinsic mechanisms to depot
variability, we studied levels of differentiation-dependent mRNAs in d
ifferentiating cultured rat epididymal and perirenal preadipocytes and
in fat cells isolated from these depots, The magnitude of the change
in levels of adipsin and glycerol-3-phosphate dehydrogenase mRNAs, whi
ch increase late during differentiation, was greater in perirenal than
epididymal preadipocytes, The magnitude of the change in beta-actin m
RNA, which decreases early during differentiation, was not sits-depend
ent, Effects of anatomic site on changes in differentiation-dependent
mRNAs observed in differentiating preadipocytes in vitro were similar
to effects of site on these mRNAs in freshly isolated fat cells: those
mRNA species whose levels increase late during preadipocyte different
iation were present in greater abundance in perirenal than epididymal
fat cells, Hence, mechanisms which underlie site-dependent variability
in adipose function may be intrinsic and could become evident midway
through the differentiation process.