C. Roche et al., TISSUE LOCALIZATION AND STAGE-SPECIFIC EXPRESSION OF THE PHOSPHOLIPIDHYDROPEROXIDE GLUTATHIONE-PEROXIDASE OF SCHISTOSOMA-MANSONI, Molecular and biochemical parasitology, 75(2), 1996, pp. 187-195
The tissue localization and the stage-specific expression of the phosp
holipid hydroperoxide glutathione peroxidase of Schistosoma mansoni (S
mPHGSHpx) have been determined. An antiserum raised against the C-term
inal region of the predicted protein sequence was used for immunocytoc
hemical investigations. The native protein is expressed only in female
and egg vitelline cells and is practically absent from male worm tiss
ue. Western blot data confirmed these results and showed the complete
absence of SmPHGSHpx from cercariae. However, Northern blotting indica
ted the presence of the corresponding mRNA at all life-cycle stages in
vestigated. The sequence determination of the 5' flanking region of th
e SmPHGSHpx gene revealed the presence of an extended TATA box (5'-TAA
ATA-3') at - 32, a possible CAAT box at - 75 and a putative monomeric
estrogen response element 5'-GGTCAA-3' at position - 486. In addition,
direct and inverted repeat elements are present.