A SULFATED CYCLODEXTRIN CHIRAL STATIONARY-PHASE FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A new sulfated beta-cyclodextrin (degree of substitution approximate t o 13-14/cyclodextrin) bonded chiral stationary phase (CSP) for high-pe rformance liquid chromatography is introduced. The novel CSP was used to resolve a number of enantiomeric pairs, including antihistamines, a ntidepressants and phenylhydantoins, using HPLC under a variety of mob ile phase conditions. Among the 33 analytes successfully enantioresolv ed, all but six had some amine functionality. Generally, the best sepa rations were obtained for analytes in which the stereogenic center was either incorporated in a ring system or positioned between two aromat ic rings. Retention seemed to arise from either inclusion complexation or electrostatic interactions. Ionic strength and pH were found to in fluence chiral recognition.