CULTURED KERATINOCYTES SEEDED ON A SILICO N-COLLAGEN-MATRIX-SHEET TO COVER FULL-THICKNESS WOUNDS

In an experimental in vitro- and in vivo-study we investigated the pro perties of a keratinocyte-seeded biomembrane. Collagen-peptides, bound ed to the undersurface of BIOBRANE(R), have a positive influence on ke ratinocyte adherence, mitosis and migration. After transplantation to nude mouse standard wounds human keratinocytes migrate towards the wou nd surface and reconstitute a neoepithelium with intact dermo-epiderma l interface. The easy, quick and cheap production and use as well as t he short culture period make MCG's a conceivable alternative to establ ished methods in the therapy of burns and chronic wounds.