AMPEROMETRIC TETRATHIAFULVALENE-MEDIATED SENSOR SENSITIVE TO REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE BASED ON CO-IMMOBILIZED LACTATE OXIDASE AND LACTATE-DEHYDROGENASE

Authors
ZHANG XL LIU HY WU XX QI DY ZHANG ZY DAI M DENG JQ FENG F
Citation
Xl. Zhang et al., AMPEROMETRIC TETRATHIAFULVALENE-MEDIATED SENSOR SENSITIVE TO REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE BASED ON CO-IMMOBILIZED LACTATE OXIDASE AND LACTATE-DEHYDROGENASE, Analytical communications, 33(3), 1996, pp. 111-114
Citations number
30
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical communications → ACNP
ISSN journal
13597337
Volume
33
Issue
3
Year of publication
1996
Pages
111 - 114
Database
ISI
SICI code
1359-7337(1996)33:3<111:ATSSTR>2.0.ZU;2-X
Abstract
An amperometric tetrathiafulvalene (TTF) mediated reduced nicotinamide adenine dinucleotide (NADH) sensor has been fabricated by co-immobili zing lactate oxidase and lactate dehydrogenase on an Eastman-AQ-TTF-mo dified electrode. The sensor provides low detection limits of 0.01 mmo l l(-1) NADH and 1.0 mu mol l(-1) L-lactate and pyruvate by means of a mplification of the bioelectrocatalytic oxidation current by the recyc ling of L-lactate and pyruvate, The response of the sensor to NADH und er N-2 saturation reaches a 95% steady-state current within 2 min with a linear response at concentrations of 0.05-2.5 mmol l(-1), The depen dence of the apparent Michaelis-Menten constant on applied potential w as studied.