THE ACCURACY CENTER OF A EUKARYOTIC RIBOSOME

Mutations in yeast ribosomal proteins and ribosomal RNAs have been sho wn to affect translational fidelity. These mutations include: proteins homologous to Escherichia coli's S4, S5, and S12; a eukaryote specifi c ribosomal protein; yeast ribosomal rRNA alterations at positions cor responding to 517, 912, and 1054 in 16S E. coli rRNA and to 2658 in th e sarcin-ricin domain of 23S E. coli rRNA. Overall there appears to be a remarkable conservation of the accuracy center throughout evolution .