APPLICATION OF ISOELECTRIC-FOCUSING IN THIN-LAYER POLYACRYLAMIDE GELSTO THE STUDY OF OPINE DEHYDROGENASES IN MARINE-INVERTEBRATES

Authors
KANNO N KAMIMURA K NAGAHISA E SATO M SATO Y
Citation
N. Kanno et al., APPLICATION OF ISOELECTRIC-FOCUSING IN THIN-LAYER POLYACRYLAMIDE GELSTO THE STUDY OF OPINE DEHYDROGENASES IN MARINE-INVERTEBRATES, Fisheries science, 62(1), 1996, pp. 122-125
Citations number
20
Categorie Soggetti
Fisheries
Journal title
Fisheries science → ACNP
ISSN journal
09199268
Volume
62
Issue
1
Year of publication
1996
Pages
122 - 125
Database
ISI
SICI code
0919-9268(1996)62:1<122:AOIITP>2.0.ZU;2-J
Abstract
Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described. The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility. It was use ful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme fo rms (allozymes and isoenzymes) in tissues of marine invertebrates.