THE EFFECT OF ETHANOL ON INTESTINAL L-LEUCINE ABSORPTION IN RATS

The chronic effect of ethanol on leucine absorption by the whole rat i ntestine (between duodenum and rectum) was studied using an in vivo mu ltiple-pass perfusion technique. Leucine concentrations in the perfusi on medium were 5, 10 and 25 mM respectively in successive passes. Etha nol was administered in drinking water during a one month induction pe riod and then for a four week period of ad libitum ingestion of 30% et hanol solution. The results were compared with ad libitum-fed control rats. The total calorie consumption due to the chow diet plus ethanol increased in the rats which had ingested ethanol when compared with th at of the controls. The daily protein intake in ethanol-fed rats was l ess than that of the controls. No significant differences in morphomet ric tissue parameters were found between the two experimental groups. Chronic ethanol ingestion provoked a slight (but not significant) decr ease in net leucine absorption at 5 mM leucine concentration. In contr ast, minor increases in the absorption values were found at 10 and 25 mM leucine concentrations. These findings suggest that the diminished active mechanisms of leucine absorption provoked by ethanol ingestion are compensated for by the enhanced diffusive processes, the passage o f the nutrients through the whole intestine, and that the low protein consumption of ethanol-fed rats in ad libitum conditions isn't enough to provoke significant decreases in leucine absorption by the whole in testine.