A. Sanfeliu et al., ANALYSIS OF NUTRITIONAL FACTORS AND PHYSICAL CONDITIONS AFFECTING GROWTH AND MONOCLONAL-ANTIBODY PRODUCTION OF THE HYBRIDOMA KB-26.5 CELL-LINE, Biotechnology progress, 12(2), 1996, pp. 209-216
The effect of medium composition and physical conditions on the growth
pattern and monoclonal antibody production of the hybridoma cell line
KB-26.5 has been studied in batch and fed-batch cultures. Different a
spects have been analyzed both individually and in combination, as it
is expected that not only one component plays a predominant role in th
is process but a combination of a number of them. Modification of the
concentrations of glucose and glutamine, supplementation of the medium
with vitamins and amino acids, influence of lactate and ammonium accu
mulation, and pulse addition of fetal calf serum have been studied in
detail, contributing to an improvement in the cell growth and IgG(3) p
roduction. Finally, the combined effect of all these factors in a fed-
batch culture leads clearly to a major improvement in the cultures, wi
th a 10-fold increase in the final monoclonal antibody concentration w
ith respect to nonoptimized batch experiments.