RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS IN HOP, HUMULUS-LUPULUS - LEVEL OF GENETIC-VARIABILITY AND SEGREGATION IN F1 PROGENY

The level of genetic variation in 24 hop genotypes was studied using t he recently developed technique for producing random amplified polymor phic DNAs (RAPDs). Of the 60 primers screened, eight produced polymorp hic RAPD bands, 38 produced bands that were monomorphic for all genoty pes and 19 did not produce any amplification product. It appeared that the level of polymorphism among the genotypes was generally low. Thre e of the primers, A11, A17 and C9, were used to determine the stabilit y and segregation of RAPD markers in five families with a total of 182 F-1 progeny. The segregation ratios of these markers in the F-1 proge ny suggested that they were inherited in a Mendelian manner. RAPD mark ers were stable and may be useful for the construction of linkage maps in hop.