SYNTENY MAPPING IN RIVER BUFFALO

The cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cel l line and river buffalo lymphocytes, by use of Southern hybridization technique. Five syntenic groups, TCRB-PGY3, ASS-ABL, FUCA1P-CRYG, MBP -YES1, and CGN1-ACTA1, previously assigned to cattle as U13, U16, U17, U28, and U29 respectively, were also found to be syntenic in buffalo. Based on the extensive syntenic conservation and banding homology bet ween cattle and river buffalo, comparative mapping predicts the locali zation of these syntenic groups on river buffalo Chromosomes (Chrs):BB U7, BBU12, BBU2q, BBU22, and BBU4q respectively as they have been prev iously localized on cattle Chrs BTA4, BTA11, BTA2, BTA24 & BTA28.