CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANT (CINC)-2-ALPHA, A NOVEL MEMBER OF RAT GRO CINCS, IS A PREDOMINANT CHEMOKINE PRODUCED BY LIPOPOLYSACCHARIDE-STIMULATED RAT MACROPHAGES IN CULTURE/
H. Nakagawa et al., CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANT (CINC)-2-ALPHA, A NOVEL MEMBER OF RAT GRO CINCS, IS A PREDOMINANT CHEMOKINE PRODUCED BY LIPOPOLYSACCHARIDE-STIMULATED RAT MACROPHAGES IN CULTURE/, Biochemical and biophysical research communications, 220(3), 1996, pp. 945-948
Neutrophil chemotactic factors (chemokines) have been purified from co
nditioned medium of lipopolysaccharide (LPS)-stimulated rat macrophage
s in culture. The LPS-stimulated macrophages produced one acidic chemo
kine, rat macrophage inflammatory protein (MIP)-1 alpha, and four basi
c chemokines, cytokine-induced neutrophil chemoattractant (CINC)-1, CI
NC-2 alpha, CINC-2 beta and CINC-3/rat MIP-2. CINC-2 alpha a novel che
mokine recently isolated from conditioned medium of rat granulation-ti
ssue culture, was the major chemoattractant among these four basic che
mokines. The results suggest that CINC-2 alpha is produced by activate
d macrophages in vivo and plays an important role in the infiltration
of neutrophils into inflammatory sites in rats. (C) 1996 Academic Pres
s. Inc.