CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANT (CINC)-2-ALPHA, A NOVEL MEMBER OF RAT GRO CINCS, IS A PREDOMINANT CHEMOKINE PRODUCED BY LIPOPOLYSACCHARIDE-STIMULATED RAT MACROPHAGES IN CULTURE/

Neutrophil chemotactic factors (chemokines) have been purified from co nditioned medium of lipopolysaccharide (LPS)-stimulated rat macrophage s in culture. The LPS-stimulated macrophages produced one acidic chemo kine, rat macrophage inflammatory protein (MIP)-1 alpha, and four basi c chemokines, cytokine-induced neutrophil chemoattractant (CINC)-1, CI NC-2 alpha, CINC-2 beta and CINC-3/rat MIP-2. CINC-2 alpha a novel che mokine recently isolated from conditioned medium of rat granulation-ti ssue culture, was the major chemoattractant among these four basic che mokines. The results suggest that CINC-2 alpha is produced by activate d macrophages in vivo and plays an important role in the infiltration of neutrophils into inflammatory sites in rats. (C) 1996 Academic Pres s. Inc.