SPECIFIC RECOGNITION OF A TETRAHEDRAL PHOSPHONAMIDATE TRANSITION-STATE ANALOG GROUP BY A RECOMBINANT ANTIBODY FAB FRAGMENT

In order to obtain antibodies able to catalyse a peptide synthesis, a naive combinatorial library of human Fab antibody fragments was screen ed with the phosphonamidate transition state analogue of the reaction. Several Fab fragments were able to bind the analogue. Competitive bin ding studies performed with molecules containing representative parts of the hapten showed that two Fabs were able to recognize specifically the tetrahedral phosphorus present in the hapten.