TURBULENT BREAKAGE OF PROTEIN PRECIPITATES IN MECHANICALLY STIRRED BIOREACTORS

Citation
Pa. Shamlou et al., TURBULENT BREAKAGE OF PROTEIN PRECIPITATES IN MECHANICALLY STIRRED BIOREACTORS, Bioprocess engineering, 14(5), 1996, pp. 237-243
Citations number
43
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
0178515X
Volume
14
Issue
5
Year of publication
1996
Pages
237 - 243
Database
ISI
SICI code
0178-515X(1996)14:5<237:TBOPPI>2.0.ZU;2-C
Abstract
Experimental data relating to the breakage of isoelectric Soya protein precipitates in a mechanically agitated bioreactor are provided and e xamined in the light of a proposed mechanistic model which relates the size of the maximum attainable aggregate diameter to the energy dissi pation rate in the vessel. The analysis suggests that protein precipit ation results in the formation of scale-invariant fractal aggregates w ith a dimensionality of 2.2. Comparing the fractal dimensionality of t he protein precipitates with reported values based on computer simulat ion studies suggests that the aggregates undergo considerable restruct uring during agitation.