A. Kapat et al., PARAMETERS OPTIMIZATION OF CHITIN HYDROLYSIS BY TRICHODERMA-HARZIANUMCHITINASE UNDER ASSAY CONDITIONS, Bioprocess engineering, 14(5), 1996, pp. 275-279
A kinetic analysis and optimization of reaction conditions for the enz
ymatic hydrolysis of chitin using chitinase produced by Trichoderma ha
rzianum NCIM 1185 was carried out. Swollen chitin was used as the subs
trate for chitinase. The central composite design was followed for thi
s optimization. The required volume ratio of the major reactants for m
aximum hydrolysis was determined. The pH and temperature optima were f
ound to be 4.75 and 47 degrees C respectively. K-m and V-max for this
enzyme were 4.643 kg/m(3) and 0.1542 U respectively.