PARAMETERS OPTIMIZATION OF CHITIN HYDROLYSIS BY TRICHODERMA-HARZIANUMCHITINASE UNDER ASSAY CONDITIONS

Authors
KAPAT A RAKSHIT SK PANDA T
Citation
A. Kapat et al., PARAMETERS OPTIMIZATION OF CHITIN HYDROLYSIS BY TRICHODERMA-HARZIANUMCHITINASE UNDER ASSAY CONDITIONS, Bioprocess engineering, 14(5), 1996, pp. 275-279
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Bioprocess engineering → ACNP
ISSN journal
0178515X
Volume
14
Issue
5
Year of publication
1996
Pages
275 - 279
Database
ISI
SICI code
0178-515X(1996)14:5<275:POOCHB>2.0.ZU;2-Z
Abstract
A kinetic analysis and optimization of reaction conditions for the enz ymatic hydrolysis of chitin using chitinase produced by Trichoderma ha rzianum NCIM 1185 was carried out. Swollen chitin was used as the subs trate for chitinase. The central composite design was followed for thi s optimization. The required volume ratio of the major reactants for m aximum hydrolysis was determined. The pH and temperature optima were f ound to be 4.75 and 47 degrees C respectively. K-m and V-max for this enzyme were 4.643 kg/m(3) and 0.1542 U respectively.