SUPERCRITICAL-FLUID CHROMATOGRAPHY OF FISH, SHARK AND SEAL OILS

Various natural and treated fish, shark liver and seal oils have been analyzed by supercritical fluid chromatography (SFC) using a non-polar capillary column. The lipids are separated according to molecular mas s. The lipid groups found included free fatty acids, cholesterol, squa lene, vitamins, wax esters, cholesterol esters, diglycerides, triglyce rides and ether lipids. Methods for the analysis of the marine oils de pend on components present in the oil. When co-eluting lipid groups we re present, modifications such as hydrogenation or TLC fractionation o f the oils had to be made. In this paper applications of SFC on fish, seal and shark liver oils are presented.