Jl. Liao et al., NOVEL SUPPORT WITH ARTIFICIALLY CREATED RECOGNITION FOR THE SELECTIVEREMOVAL OF PROTEINS AND FOR AFFINITY-CHROMATOGRAPHY, Chromatographia, 42(5-6), 1996, pp. 259-262
Citations number
6
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Acrylamide and N,N'-methylenebisacrylamide were copolymerized in the p
resence of a protein to form a gel which was pressed through a sieve.
The gel particles obtained were packed into a chromatographic tube. Th
e experimental conditions for the polymerization are such that the por
es of the gel particles are large enough to permit the protein to diff
use out of the particles, so that the entrapped protein can be removed
from the bed by washing with an aqueous solution. However, the intera
ction with the matrix is so strong that the protein can be desorbed on
ly by a buffer containing 0.5 M sodium chloride or by a 10 % solution
of acetic acid containing 10 % SDS. When a sample containing the prote
in present during the polymerization was applied to the column along w
ith other proteins this protein was the only one adsorbed. The techniq
ue worked selectively with hemoglobin, cytochrome C and transferrin.