CHIRAL SEPARATION OF POLYCHLORINATED-BIPHENYLS BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH GAMMA-CYCLODEXTRIN AS MODIFIER IN THE SEPARATION BUFFER

Cyclodextrin-modified micellar electrokinetic chromatography has enabl ed the chiral separation of polychlorinated biphenyls (PCBs). Twelve P CBs (45, 84, 88, 91, 95, 132, 136, 139, 149, 171, 183 and 196) were in dividually separated into their two enantiomers by using gamma-cyclode xtrin as modifier in a separation buffer containing 2-(N-cyclohexylami no)ethanesulphonic acid (CHES) and sodium dodecyl sulphate (SDS) micel les. Multicomponent separations were also performed. The separation of the eighteen enantiomers of a mixture of nine chiral PCBs has been pe rformed in a short analysis time (ca. 35 min).