Parentage control has been performed for 15 litters from 12 different
dog breeds by amplification of microsatellites. As it was possible to
include all putative parents in all cases, they were solved by exclusi
on. Discrimination between parents/non-parents was made after genotypi
ng of 6-9 microsatellite loci. In 12 of the cases all but one of the a
lleged fathers were excluded while in three cases it was unambiguously
shown that superfecundation had taken place. Furthermore, one inclusi
on case concerning disputed maternity has been investigated. Maternity
indices were calculated for 12 loci and probability of maternity was
estimated to be 99.99%. These results testify that microsatellites can
be applied very efficiently for resolution of parentage in dogs.