R. Lisin et al., TIME-DOMAIN DIELECTRIC-SPECTROSCOPY STUDY OF HUMAN-CELLS .1. ERYTHROCYTES AND GHOSTS, Biochimica et biophysica acta. Biomembranes, 1280(1), 1996, pp. 34-40
A method, allowing correction of electrode polarization effect in case
of cell suspensions of small volume fraction is proposed. The dielect
ric behavior of human erythrocytes and erythrocyte ghosts suspensions
was studied by time domain dielectric spectroscopy (TDDS) and the esti
mation of the dielectric constants of cell's structural parts (membran
e, cytoplasm) on the basis of suitable models are presented. It was sh
own that in the case of small volume fraction of cells in suspension,
the electrode polarization effect can be taken into account by the add
itional measurement of the corresponding supernatant. The optimal volu
me fraction of cells in suspensions in the TDDS measurements was found
to be 3%-10%. The erythrocyte and erythrocyte ghost suspensions were
found to demonstrate a single dispersion which can be described by the
Debye equation. The membrane dielectric constant of different erythro
cytes and ghosts was distributed near 5.