QUALITATIVE ASSESSMENT OF INTRATHECAL IGG SYNTHESIS BY ISOELECTRIC-FOCUSING AND IMMUNODETECTION - INTERLABORATORY REPRODUCIBILITY AND INTEROBSERVER AGREEMENT
F. Sellebjerg et M. Christiansen, QUALITATIVE ASSESSMENT OF INTRATHECAL IGG SYNTHESIS BY ISOELECTRIC-FOCUSING AND IMMUNODETECTION - INTERLABORATORY REPRODUCIBILITY AND INTEROBSERVER AGREEMENT, Scandinavian journal of clinical & laboratory investigation, 56(2), 1996, pp. 135-143
Detection of intrathecal IgG synthesis is important in patients with s
uspected multiple sclerosis (MS). The recommended method for the detec
tion of intrathecal synthesis of IgG is isoelectric focusing and immun
odetection of oligoclonal bands. Recently ''The Committee for European
Concerted Action for Multiple Sclerosis'' has recommended that the re
sults of isoelectric focusing for the detection of intrathecal synthes
is of oligoclonal bands should not only be stated as positive or negat
ive for intrathecal synthesis; instead, the laboratory should provide
a detailed description of the IgG pattern in both cerebrospinal fluid
(CSF) and serum together with a conclusion concerning the presence of
intrathecal synthesis. We studied the interlaboratory reproducibility
and interobserver agreement of isoelectric focusing, and the recommend
ed classification system for the assessment of intrathecal IgG synthes
is, in two separate patient groups employing kappa statistics. We foun
d a high degree of interlaboratory reproducibility (133 patients; kapp
a = 0.95 +/- 0.05) and interobserver agreement (356 patients; kappa =
0.97 +/- 0.04) when the presence or absence of intrathecal IgG synthes
is was assessed. The agreement was less pronounced, although still ful
ly satisfactory, when the results were classified according to the det
ailed system by two laboratories (133 patients; kappa = 0.86 +/- 0.08)
and two observers (356 patients; kappa = 0.88 +/- 0.08). Two specific
problems in the interpretation of isoelectric focusing patterns were
identified: one related to the discrimination of a pattern with severa
l closely spaced bands which may represent a monoclonal protein; the o
ther related to determining whether systemic band synthesis was presen
t. We conclude that isoelectric focusing and immunodetection is a very
reproducible technique for the detection of intrathecal Ige synthesis
. Well defined criteria and extensive standardization may, however, be
necessary when more elaborate classification systems are employed.