OXIDATIVE DAMAGE TO LENS MEMBRANES INDUCED BY METAL-CATALYZED SYSTEMS

The oxidative effect of hydrogen peroxide, ascorbic acid and glucose i n the presence of transition metals was studied. Incubation of bovine lens membranes with hydrogen peroxide/metal, ascorbic acid/metal and g lucose/metal systems resulted in a significant augmentation of thiobar bituric acid-reactive substance content in the membranes. Presence of alpha-tocopherol decreased the extent of oxidative damage. This oxidat ion was found to be mediated by hydroxyl radicals. Dependence of hydro xyl radical generation upon the buffer used has been observed. In all experiments Cu(II) ions exhibited a higher efficiency compared to Fe(I I) ions.