CHARACTERIZATION OF A CDNA AND GENE ENCODING THE MOUSE THETA-CLASS GLUTATHIONE TRANSFERASE MGSTT2 AND ITS LOCALIZATION TO CHROMOSOME 10B5-C1

In this study, we have isolated and characterized a gene and cDNA enco ding a mouse Theta class GST. The gene, mGSTT2, spans approximately 3. 1 kb and is composed of five exons interrupted by four introns. The ge ne was localized to Chromosome 10B5-C1 by in situ hybridization. South ern blot analysis of mouse genomic DNA suggests that there is only one copy of mGSTT2 in the mouse genome. The cDNA derived from mGSTT2 was isolated from a mouse liver cDNA library and has an open reading frame of 732 bp encoding a peptide of 244 amino acids with a calculated mol ecular weight of 26,676 Da. The encoded protein shares amino acid sequ ence identities of 92, 77, 51, and 55% with rat subunit Yrs, human sub unit GSTT2, rat subunit 5, and human subunit GSTT1, respectively. (C) 1996 Academic Press, Inc.