CLONING OF THE HUMAN SIX1 GENE AND ITS ASSIGNMENT TO CHROMOSOME-14

The recently described murine homeobox genes, Six1 and Six2, which are expressed during development in limb tendons, have also been shown to be expressed in skeletal and smooth muscle, respectively, We have clo ned and sequenced a human SIX1 cDNA and shown by Northern blotting tha t it is expressed in adult skeletal muscle. The cDNA sequence and pred icted protein sequence of SIX1 and Six1 are highly homologous, with 98 % similarity over the entire predicted amino acid sequence, SIX1 was m apped to human chromosome 14 using a rodent/human somatic cell hybrid panel. (C) 1996 Academic Press, Inc.