Y. Shacharhill et al., MEASURING NITRATE IN PLANT-CELLS BY IN-VIVO NMR USING GD3-REAGENT( ASA SHIFT), Journal of magnetic resonance. Series B, 111(1), 1996, pp. 9-14
NMR investigations of nitrate in plant cells and tissues have hitherto
been limited by the indistinguishability of the signals from intracel
lular and extracellular nitrate. Gd3+ is shown to be an effective shif
t reagent for N-14 and N-15 nitrate NMR signals, resolving the interna
l and external nitrate signals in plant tissues, including cell suspen
sions and root material. However, time-course experiments show that, w
hile the use of Gd3+ allows nitrate levels to be monitored over extend
ed periods, it also has adverse effects on growth and nitrate uptake.
Accordingly, a number of chelated forms of gadolinium were investigate
d, and it is concluded that the NMR contrast agent Gd(DTPA-BMA) is lik
ely to be a suitable shift reagent for physiologically relevant studie
s of nitrate transport in roots. (C) 1996 Academic Press, Inc.