SUBCELLULAR-LOCALIZATION OF ALEUTIAN MINK DISEASE PARVOVIRUS PROTEINSAND DNA DURING PERMISSIVE INFECTION OF CRANDELL FELINE KIDNEY-CELLS

Confocal microscopy allowed us to localize viral nonstructural (NS) an d capsid (VP) proteins and DNA simultaneously in cells permissively in fected with Aleutian mink disease parvovirus (ADV). Early after infect ion, NS proteins colocalized with viral DNA to form intranuclear inclu sions, whereas VP proteins formed hollow intranuclear shells around th e inclusions. Later, nuclei had irregular outlines and were virtually free of ADV products. In these cells, inclusions of viral DNA with or without associated NS protein were embedded in cytoplasmic VP protein. These findings implied that ADV replication within an infected cell i s regulated spatially as well as temporally.