CHARACTERIZATION OF INDEPENDENT DNA AND MULTIPLE ZN-BINDING DOMAINS AT THE N-TERMINUS OF HUMAN DNA-(CYTOSINE-5) METHYLTRANSFERASE - MODULATING THE PROPERTY OF A DNA-BINDING DOMAIN BY CONTIGUOUS ZN-BINDING MOTIFS
Lsh. Chuang et al., CHARACTERIZATION OF INDEPENDENT DNA AND MULTIPLE ZN-BINDING DOMAINS AT THE N-TERMINUS OF HUMAN DNA-(CYTOSINE-5) METHYLTRANSFERASE - MODULATING THE PROPERTY OF A DNA-BINDING DOMAIN BY CONTIGUOUS ZN-BINDING MOTIFS, Journal of Molecular Biology, 257(5), 1996, pp. 935-948
We report here a detailed mapping and characterisation of a DNA-bindin
g domain at the N terminus of human DNA-(cytosine-5) methyltransferase
. A small region, B1 (codon 202 to 369), was first identified by its Z
n- and gross DNA-binding properties. Further fine-mapping using deleti
on and point mutation analysis shows that the DNA- and Zn-binding doma
ins involve separate peptide motifs, (K) under bar (R) under bar (R) u
nder bar (K) under bar TTPKEP (T) under bar (E) under bar (K) under ba
r (K) under bar (codons 202 to 215) for a bipartite DNA-binding oligop
eptide (DB1) and CX(2)CX(13)HX(2) D(X)(23)EX(2)EX(13)CX(3)H (codons 23
2 to 297) for possibly two contiguous Zn-binding domains (AZn), which
can function independently However, B1 (containing DB1 and AZn) differ
s from DB1 because it does not bind to a 30 base-pair duplex. Interest
ingly, H3 codons 202 to 974, which encloses B1 and B2 (containing the
Zn-binding CX(2)CX(2)CX(4)CX(2)CX(2)C motif from codon 533 to 550) bin
ds preferentially to 0.8 kb duplexes, as compared with 0.4 and 0.6 kb
duplexes. As the homologous murine B1, which targets the murine methyl
ase to replication foci, also binds to DNA and Zn, it is possible that
the N terminus of mammalian methylase may be involved in sensing the
appropriate length of newly synthesized DNA before methylation by its
C terminus. This may enable a time delay for the transient existence o
f hemi-methylation sites for their unknown biological functions in mam
mals. (C) 1996 Academic Press Limited