EFFICIENT ISOLATION METHOD FOR HUMAN RECO MBINANT TUMOR-NECROSIS-FACTOR-ALPHA

Authors
TIKHONOV RV YAKIMOV SA KOROBKO VG WULFSON AN
Citation
Rv. Tikhonov et al., EFFICIENT ISOLATION METHOD FOR HUMAN RECO MBINANT TUMOR-NECROSIS-FACTOR-ALPHA, Bioorganiceskaa himia, 22(3), 1996, pp. 163-167
Citations number
11
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
22
Issue
3
Year of publication
1996
Pages
163 - 167
Database
ISI
SICI code
0132-3423(1996)22:3<163:EIMFHR>2.0.ZU;2-6
Abstract
An efficient and productive isolation method for human recombinant tum or necrosis factor a from Escherichia coil cells was developed. The me thod includes a membrane filtration step, two steps of ion-exchange ch romatography, and gel filtration on a Sephadex G-25 column. The target product was obtained with approximately 50% total yield and greater t han 95% purity according to PAGE and HPLC.