ARTIFICIAL GENE, BIOSYNTHESIS, AND PROPER TIES OF HUMAN-IMMUNOGLOBULIN G1 FC FRAGMENT

Authors
EFIMOV VA KALINKINA AL FRADKOV AF CHAKHMAKHCHEVA OG
Citation
Va. Efimov et al., ARTIFICIAL GENE, BIOSYNTHESIS, AND PROPER TIES OF HUMAN-IMMUNOGLOBULIN G1 FC FRAGMENT, Bioorganiceskaa himia, 22(3), 1996, pp. 168-174
Citations number
16
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
22
Issue
3
Year of publication
1996
Pages
168 - 174
Database
ISI
SICI code
0132-3423(1996)22:3<168:AGBAPT>2.0.ZU;2-N
Abstract
Chemicoenzymatic synthesis and cloning of a gene encoding the Fc domai n of human immunoglobulin G1 were carried out. The artificial gene was expressed in Escherichia coli cells in plasmid vectors under control of a late T7 promoter. The recombinant protein isolated from the bacte rial cells is capable of forming dimers and binding protein A from Sta phylococcus aureus.