For the study of the antigenic structure and function of human interle
ukin 2, the peptides corresponding to its 60-72 sequence were synthesi
zed by conventional methods of peptide chemistry in solution. To enhan
ce the stability of the synthetic peptides towards the proteolysis and
to remove their terminal charges, we acetylated their NH2 groups and
esterified with methanol their carboxyls. Some of these peptides were
converted from being cytotoxic to possessing strong growth-stimulating
activity for the preliminaryly activated macrophages both in vitro an
d in vivo. The biologically active peptides were also shown to enhance
regeneration-reparation processes in liver and skin.