ASSAY OF FREE D-BIOTIN - COMPARISON OF IN STRUMENTAL AND NONINSTRUMENTAL METHODS

Authors
PAVLOVA IS LYUBAVINA IA LUKIN YV TSIBULSKAYA MI KOROLEV PN
Citation
Is. Pavlova et al., ASSAY OF FREE D-BIOTIN - COMPARISON OF IN STRUMENTAL AND NONINSTRUMENTAL METHODS, Bioorganiceskaa himia, 22(3), 1996, pp. 223-227
Citations number
14
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
22
Issue
3
Year of publication
1996
Pages
223 - 227
Database
ISI
SICI code
0132-3423(1996)22:3<223:AOFD-C>2.0.ZU;2-5
Abstract
Simple methods for determining free d-biotin were developed based on t he latex agglutination reaction inhibition and competitive ELISA. Biot in-binding protein Str used as a biospecific marker was immobilized on the surface of colored polyacrolein latex particles or a nitrocellulo se membrane. The sensitivity of both methods of the free d-biotin assa y, which take 1.5 h, is 1.0-3.0 ng/ml.