REAL-TIME 2-PHOTON CONFOCAL MICROSCOPY USING A FEMTOSECOND, AMPLIFIEDTI-SAPPHIRE SYSTEM

Authors
BRAKENHOFF GJ SQUIER J NORRIS T BLITON AC WADE MH ATHEY B
Citation
Gj. Brakenhoff et al., REAL-TIME 2-PHOTON CONFOCAL MICROSCOPY USING A FEMTOSECOND, AMPLIFIEDTI-SAPPHIRE SYSTEM, Journal of Microscopy, 181, 1996, pp. 253-259
Citations number
16
Categorie Soggetti
Microscopy
Journal title
Journal of Microscopy → ACNP
ISSN journal
00222720
Volume
181
Year of publication
1996
Part
3
Pages
253 - 259
Database
ISI
SICI code
0022-2720(1996)181:<253:R2CMUA>2.0.ZU;2-Z
Abstract
The bilateral imaging approach known from confocal applications operat ing in the line mode was used to realize real-time two-photon imaging. It is shown that the sectioning inherent to two-photon imaging could be improved by the introduction of a confocal lie aperture in the imag ing path. Using a high-power, low-repetition-rate amplified Ti:sapphir e system, various biological objects were visualized including live bo ar sperm.