CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC STUDIES OF L-2-HALOACID DEHALOGENASE FROM PSEUDOMONAS SP. YL

The dimeric L-2-haloacid dehalogenase from Pseudomonas sp. YL, (subuni t mass, 26179 Da), has been crystallized by vapor diffusion, supplemen ted by repetitive seeding, against a 50 mM potassium dihydrogenphospha te solution (pH 4.5) containing 15% (w/v) polyethylene glycol 8,000 an d 1% (v/v) n-propanol, The crystals belong to the monoclinic space gro up C2 with unit cell dimensions of a = 92.21 Angstrom, b = 62.78 Angst rom, c = 50.84 Angstrom, and beta = 122.4 degrees, and contain two deh alogenase dimers in the unit cell, They are of good quality and diffra ct up to 1.5 Angstrom resolution. (C) 1996 Wiley-Liss, Inc.