P. Mahendra et al., PERIPHERAL-BLOOD PROGENITOR-CELL TRANSPLANTATION - A SINGLE-CENTER EXPERIENCE COMPARING 2 MOBILIZATION REGIMENS IN 67 PATIENTS, Bone marrow transplantation, 17(4), 1996, pp. 503-507
Between June 1991 and January 1995 we performed 67 peripheral blood pr
ogenitor cell transplants (PBPCT), Ten patients (group 1) were mobilis
ed with 7 gm/m(2) of cyclophosphamide followed by daily G-CSF injectio
ns (5 mu g/kg, subcutaneously). When the white cell count reached 1 x
10(9)/l they were leukapheresed for 5 days, After stem cell infusion t
hey received G-CSF (10 mu g/kg/day) until the neutrophil count reached
1.5 x 10(9)/l, Fifty-six patients had PBPCs mobilised with 3 gm/m(2)
of cyclophosphamide followed by daily subcutaneous G-CSF (5 mu g/kg) a
nd PBPCs were harvested on 2 consecutive days, when the white cell cou
nt rose to 4 x 10(9)/l, After stem cell infusion this group did not re
ceive G-CSF, In 47 of the 56 patients (group 2) adequate MNC (greater
than or equal to 4 x 10(8)/kg) and/or CFU-GM (greater than or equal to
10 x 10(4)/kg) were obtained, Insufficient MNC and/or CFU-GM were obt
ained in 10 patients, They were therefore transplanted using a combina
tion of bone marrow and peripheral blood progenitor cells (group 3), O
verall 64 patients successfully engrafted, Median days to neutrophils
greater than or equal to 0.5 x 10(9)/l were 9 (range 8-13), 12 (range
8-25) and 11 (range 9-16) and to platelets greater than or equal to 50
x 10(9)/l were 11 (range 9-23), 13 (range 9-90) and 16 (range 13-99)
in groups 1, 2 and 3 respectively, Patients in group 1 had a faster ne
utrophil recovery than patients in group 2 (P = 0.0002), The three pat
ients who failed to engraft all received a combination of autologous p
eripheral blood and bone marrow cells.