TACROLIMUS (FK506) AND METHOTREXATE REGIMENS TO PREVENT GRAFT-VERSUS-HOST DISEASE AFTER UNRELATED DOG LEUKOCYTE ANTIGEN (DLA) NONIDENTICAL MARROW TRANSPLANTATION

We previously reported on synergism between methotrexate and tacrolimu s (FK506) in preventing graft-versus-host disease (GVHD) in dogs given DLA-nonidentical unrelated marrow grafts after 9.2 Gy of total body i rradiation (TBI), Methotrexate was given at 0.4 mg/kg i.v. on days 1, 3, 6 and 11 and FK506 at 0.15 mg/kg/day i.m. on days 0-8 and 0.5 mg/kg /day orally on days 9-90, Half of the dogs became long-term survivors, A major toxicity was gastrointestinal, and 25% of dogs died with intu ssusception, The current study addresses the problem of intussusceptio n by making changes in drug doses used, In one group of dogs, FK506 wa s reduced to 0.075 mg/kg i.m. on days 1-8, while methotrexate was admi nistered per original schedule, In a second group, methotrexate was re duced to a single dose on day 7, while FK506 was either administered p er the original or reduced-dose schedule, None of the 17 current dogs developed intussusception, however, all but two dogs died with GVHD (n = 12) or graft failure (ii = 3), Only two dogs survived after transie nt GVHD, Results show that there is little room for maneuvering FK506 or methotrexate doses, and hopes of reducing gastrointestinal toxicity by dose modifications while retaining the ability to prevent GVHD wer e not fulfilled.