DETECTION OF EPSTEIN-BARR-VIRUS GENOME IN OCULAR-TISSUES

Background: Epstein-Barr virus (EBV) is a ubiquitous mucosal pathogen with a propensity for lifelong, asymptomatic persistence. Because of r eported associations between EBV and ocular inflammatory disorders, we tested ocular tissues from normal eyes for presence of the EBV genome . Methods: Ten freshly harvested cadaveric human eyes were dissected i nto limbal cornea, central cornea, aqueous humor, iris, vitreous humor , and optic nerve. Total cellular DNA preparations were screened for D NA sequences specific to EBV's large internal repeat region. After Sou thern transfer, polymerase chain reaction products were detected by a P-32-labeled oligonucleotide probe specific to amplified sequences int ernal to the polymerase chain reaction primers. Results: Seven of ten eyes from deceased donors yielded a polymerase chain reaction product, indicating presence of EBV genome. In all, 12 (20%) of 60 cadaveric o cular samples contained EBV DNA. Only the optic nerve was consistently negative for EBV DNA. Conclusions: Detection of EBV DNA in cadaveric ocular tissues indicates a broad anatomic distribution of this persist ent mucosal pathogen. The frequency with which EBV was found at appare ntly normal ocular sites raises the possibility for viral involvement in disease states, but emphasizes the need for specific criteria to im plicate EBV in ocular pathology.