PERFORMANCE OF GLUCOSE BIOSENSOR BASED ON OXYGEN-ELECTRODE IN PHYSIOLOGICAL FLUIDS AND AT BODY-TEMPERATURE

A potentially implantable glucose biosensor for continuous monitoring of glucose levels in diabetic patients has been developed. The glucose biosensor is based on an amperometric oxygen electrode and glucose ox idase immobilized on carbon powder held in the form of a liquid suspen sion. The enzyme material can be replaced (the sensor recharged) witho ut sensor disassembly. Diffusion membranes made of silastic coatings o ver a microporous polycarbonate membrane are used. Calibration curves of the sensors in phosphate buffer solution and in undiluted blood pla sma at body temperature have been obtained. The reproducibility of the sensor response in serum at body temperature is demonstrated. The sen sors have a stable signal during storage and continuous operation at b ody temperature for a period of at least one month.