SEROLOGICAL AND GENOMIC CHARACTERIZATION OF PORCINE ROTAVIRUSES IN THAILAND - DETECTION OF A G10 PORCINE ROTAVIRUS

A total of 557 fecal specimens collected from piglets with diarrhea in Thailand were examined for rotavirus RNA by polyacrylamide gel electr ophoresis. Twenty-three, one, and two samples were positive for group A, group B, and group C rotaviruses, respectively. Two samples exhibit ed two segments found in picobirnavirus RNA. RNA electropherotyping of 23 group A rotaviruses showed that they were classified into five pat terns. By serotyping by enzyme-linked immunosorbent assay and PCR, vir uses in 3 and 14 specimens were found to be serotype G3 and serotype G 10, respectively. For one specimen, containing a serotype G10 virus (s train P343), virus was isolated in MA-104 cells, and the nucleotide se quences of the VP7 and VP4 genes were determined. Comparative sequence analysis and cross-neutralization tests showed that strain P343 has B 223-like G10 and UK-like P7 serotype (or VP4 genotype 5) specificities . Rotaviruses having such antigenic specificities have not been detect ed in piglets. Thus, the interspecies transmission of rotaviruses betw een cows and pigs was suggested.