G. Dobson et Cl. Lee, IMPROVED METHOD OF DETERMINING THE SEX OF THE MARINE TOPSHELL, TROCHUS-NILOTICUS (MOLLUSCA, GASTROPODA) FOR SPAWNING, Aquaculture, 139(3-4), 1996, pp. 329-331
Determining the sex of Trochus niloticus for induced spawning is extre
mely difficult. A quick and simple method for therefore developed thro
ugh a process involving cutting a 'window' in the shell until the nacr
e layer was revealed. Through the window, the color of the gonad could
be seen and the sex of the animal determined - dark green in the case
of a female and creamy white in the case of a male. The method did no
t appear to inflict obvious stress nor mortality on the treated animal
s. Successful spawning inductions were achieved by using this method o
f determining the sex of the broodstock.