SYNTHESIS OF ALANINE AND LEUCINE BY REDUCTIVE AMINATION OF 2-OXOIC ACID WITH COMBINATION OF HYDROGENASE AND DEHYDROGENASE

Authors
HASUMI F FUKUOKA K ADACHI S MIYAMOTO Y OKURA I
Citation
F. Hasumi et al., SYNTHESIS OF ALANINE AND LEUCINE BY REDUCTIVE AMINATION OF 2-OXOIC ACID WITH COMBINATION OF HYDROGENASE AND DEHYDROGENASE, Applied biochemistry and biotechnology, 56(3), 1996, pp. 341-344
Citations number
4
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
56
Issue
3
Year of publication
1996
Pages
341 - 344
Database
ISI
SICI code
0273-2289(1996)56:3<341:SOAALB>2.0.ZU;2-9
Abstract
Alanine synthesis by reductive amination of pyruvate was performed by the combination of NADH regeneration system and alanine dehydrogenase (AlaDH). The conversion of pyruvate to alanine was 99% after 1 h. Leuc ine synthesis was also carried out by the combination of NADH regenera tion system and leucine dehydrogenase (LeuDH). The conversion of 4-met hyl-2-oxovalerate to leucine was 60% after 1.5 h.