SOME ASPECTS OF SPECTROPHOTOMETRIC DETERMINATION OF AMINO-ACIDS USINGO-PHTHALALDEHYDE AS A CHROMOGENIC REAGENT

Authors
AMINUDDIN M SHAH MA CHOHAN ZH
Citation
M. Aminuddin et al., SOME ASPECTS OF SPECTROPHOTOMETRIC DETERMINATION OF AMINO-ACIDS USINGO-PHTHALALDEHYDE AS A CHROMOGENIC REAGENT, Journal of the Chemical Society of Pakistan, 18(1), 1996, pp. 8-11
Citations number
15
Categorie Soggetti
Chemistry
Journal title
Journal of the Chemical Society of Pakistan → ACNP
ISSN journal
02535106
Volume
18
Issue
1
Year of publication
1996
Pages
8 - 11
Database
ISI
SICI code
0253-5106(1996)18:1<8:SAOSDO>2.0.ZU;2-U
Abstract
A rapid, sensitive and convenient spectrophotometric method has been d eveloped for the determination of Amino acids. At lower concentration (3 x 10(-7) mol/ml) of amino acids the Beer-Lambert law is obeyed ?he absorptivities of me o-phthalaldehyde (OPA)-adducts formed with differ ent amino acids have been examined and calculated The OPA-derivative i s found to be composed of amino acid, OPA and a thiol in a molar ratio 1:2:1.