Primiparous dairy cows were utilized in two experiments to evaluate th
e productive benefits of modifying the strategy of within-day feeding
of soybean meal (SBM) as a supplement to a basal mixed ration fed twic
e daily. The design of the two experiments was the same, although one
was completed with early-lactation cows and the other with late-lactat
ion cows. The evaluation of protein status of all treatments, after th
e experiments were complete, indicated that cows were limited by suppl
ies of digestible undegraded intake protein (DUIP) on all treatments o
f both experiments. However, benefits of feeding 800 g d(-1) of supple
mental SBM versus none were primarily restricted to enhanced productio
n of milk protein, and it is suggested that this was primarily the res
ult of the contribution of DUIP from SBM to intestinal protein supply.
Feeding SBM in two versus four daily meals either 1 or 1 and 5 h, res
pectively, after offer of the mixed ration had no influence on animal
performance at either stage of lactation. However, feeding SBM in two
daily meals 1 h prior to the mixed ration versus in two daily meals 1
h after offer of the mixed ration enhanced milk, milk energy, and milk
lactose output in late lactation, but not in early lactation. These a
pparently inconsistent production responses between stages of lactatio
n are consistent with a hypothesis that microbial growth is progressiv
ely restricted as supplies of degraded intake protein (DIP), relative
to requirements, decline. Under these conditions, which applied to the
late-lactation cows, feeding DIP at times of the day when rumen-solub
le N levels would be lowest (i.e., pre-feeding) would be beneficial fo
r stimulating microbial growth prior to ingestion of the mixed ration.