STUDIES OF HUMAN AND RAT-BLOOD UNDER OXIDATIVE STRESS - CHANGES IN PLASMA THIOL LEVEL, ANTIOXIDANT ENZYME-ACTIVITY, PROTEIN CARBONYL CONTENT, AND FLUIDITY OF ERYTHROCYTE-MEMBRANE

previously by selection and inbreeding of Wistar rats susceptible or r esistant to the cataractogenic effect of galactose the S and R rat str ains were developed as a model of oxidative stress. It was earlier fou nd S rats have elevated level of OH-radical generation and enhanced li pid peroxidation compared with that for R rats. Our data show that sup eroxide dismutase and catalase activities in the blood of S mts are tw ice lower than corresponding values of R rats. Increased level of disu lfides and protein carbonyl groups was obtained in the blood plasma of S-rats. It was found that fluidity of erythrocyte membrane measured:b y photobleaching method is definitely higher for S rats compared with that for S rats. Similar effect of decreased catalase activity and inc reased disulfide content has been observed for kyphoscoliosis patients . (C) 1996 Academic Press, Inc.