M. Sandberg et J. Meijer, STRUCTURAL CHARACTERIZATION OF THE HUMAN SOLUBLE EPOXIDE HYDROLASE GENE (EPHX2), Biochemical and biophysical research communications, 221(2), 1996, pp. 333-339
The structural organization of the human gene encoding soluble epoxide
hydrolase (EPHX2) was determined. Cosmid clones containing the EPHX2
gene were identified by hybridization of a human genomic library with
PCR amplified cDNA to the mouse or human soluble epoxide hydrolase as
probes. The gene consists of 19 exons and is approximately 45 kb of wh
ich more than 15 kb were sequenced. The coding sequence corresponds to
555 amino acid residues, i.e., one additional residue to those in the
reported cDNA sequence. The sizes of the introns were defined by sequ
encing or PCR analysis and they contained various repetitive structure
elements as expected. (C) 1996 Academic Press, Inc.