COMPARATIVE H-1-NMR STUDIES ON THE PHYSICAL STATE OF WATER IN SOFT CONTACT-LENS AND MOUSE LENS

Citation
K. Kuwata et al., COMPARATIVE H-1-NMR STUDIES ON THE PHYSICAL STATE OF WATER IN SOFT CONTACT-LENS AND MOUSE LENS, Biochimica et biophysica acta (G). General subjects, 1289(3), 1996, pp. 369-376
Citations number
38
Categorie Soggetti
Biology,Biophysics
ISSN journal
03044165
Volume
1289
Issue
3
Year of publication
1996
Pages
369 - 376
Database
ISI
SICI code
0304-4165(1996)1289:3<369:CHSOTP>2.0.ZU;2-M
Abstract
The physical state of water in mouse lenses (2-, 4- or 8-wk-old) and s oft contact lenses (SCLs, water content from 18.4 to 79.2%) were studi ed by measuring spin-lattice relaxation times (T-1) and apparent inter molecular cross-relaxation times (T-IS) from irradiated protein or pol ymer protons to water protons, using 360 MHz H-1-NMR spectrometer at 2 5 degrees C. (1) 1/T-1 values of SCLs increased gradually with increas ing dry weight (W(%)). 1/T-IS values of SCLs were approximately zero a t W of 20.8 and 26.8%, increased gradually from 26.8% and then steeply above similar to 50%. (2) A plot of 1/T-1 vs. W(%) of mouse lenses wa s almost equal to that of SCLs. However, a plot of 1/T-IS vs. W(%) was an approximately straight line with the intercept at W of 23% and wit h the slope which is almost equal to that of SCLs above W of similar t o 50%. The plot of 1/T-IS vs. W(%) of mouse lenses might indicate the significant change in the physical state of water and/or protein-water interactions above W of 23%.