OSCILLATIONS IN THE PEROXIDASE-OXIDASE REACTION - A COMPARISON OF DIFFERENT PEROXIDASES

The nonlinear behavior of the peroxidase-oxidase reaction was studied using structurally different peroxidases. For the first time sustained oscillations with peroxidases other than horseradish peroxidase in a single-enzyme system were observed. All peroxidases that showed signif icant oxidase activity were able to generate sustained oscillations. W hen adjusting the overall reaction rate, either of the two modifiers 2 ,4-dichlorophenol or Methylene blue could be omitted from the reaction . Due to the observation of different enzyme intermediates when using different peroxidases, we conclude that the mechanisms responsible for oscillatory kinetics may vary from one peroxidase to the other.